Assayfit Pro universal online curve fitting and assay data calculation

online assay results calculator

Perform curve fitting and calculation of laboratory tests using this page using AssayFit Pro version 5.3.1 Click here to read the terms for use and distribution.
Alternatively use the Microsoft Excel add-in to perform data analysis. Previous version 5.2. Check the menu for other online options and previous versions.
For Research Use Only. Not for use in diagnostic procedures.

Data entry

1. Enter your assay result data in the spreadsheet like tables below, you can copy and paste from Excel.
2. Enter calibrator data in the table or indicate position of the calibrators in the sample list table.
Select a region and click the CAL button to fill the region with a dilution series of the calibrators. You can also indicate the number of replicates.
3. Click the "cals from sample list" button to copy the calibrator values from the sample table.
4. Indicate the position of samples, controls and blanks by selecting the cells and clicking the S, C and B buttons
5. Enter the run Id and select the curve fitting function.
6. Optional: you can subtract blank values or normalize, select this from the options menu.
7. Click submit to perform curve fitting and data analysis.

Unknown samples

Run ID

Curve fit settings

Function

Server

Key

Assay calculation results

Fit Summary

Calibration curve

Results

Individual results

Fit output