Assayfit Pro ELISA online curve fitting

ELISA assay results data analysis

Perform curve fitting with Assayfit Pro using this page. You can use this service for free 10 times each day. Click here to read the terms for use and distribution.
Alternatively use the Microsoft Excel add-in to perform data analysis. Check the menu for other online options and previous versions.
For Research Use Only. Not for use in diagnostic procedures.

ELISA layout and sample IDs

Enter concentration values for the calibrator, select region and click toggle calibrators button. You can also enter Blank, Control and sample IDs. Sample IDs are kept on your local computer and are not sent over the internet.

Sample dilution

Curve fit input values

You can copy the values from Excel, enter them manually or use the 'Cals from layout' button to copy all calibrator values marked with CAL- from the layout.

Curve fit settings




Run ID

ELISA data analysis results

Fit Summary

Calibration curve



Sample ID




Conc x Dilution

Fit output